Phospho-erk1/2 thr202/tyr204
WebSpecificity / Sensitivity. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when … WebOct 31, 2014 · In the ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, anti-Phospho-ERK1/2 (Thr202/Tyr204) or anti-ERK1/2 is pipetted into the wells and incubated.
Phospho-erk1/2 thr202/tyr204
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WebWhole cell extracts (15 μg protein) from serum-starved Raw264.7 cells untreated (-) or treated (+) with 100 ng/mL PMA for 1 hour were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone Poly21A07) for 2 hours at room temperature. WebAnti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody (GTX24819) GeneTex Back Primary Antibodies Secondary Antibodies Antibody Panels ELISA Antibody Pairs & Kits Isotype Controls Proteins & Peptides Slides Lysates Serums & Plasmas Reagents Research Kits Research Tools Back Cancer Tumor Markers …
WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 10404 publications. Validated for WB, IP, IHC, IF, F. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit Monoclonal Antibody (CST #4370) is ready to … WebHome › ERK1/2 (Thr202 + Tyr204) Antibody Lane 1: human colon carcinoma lysates probed with Anti phospho-ERK1\/2 (Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 44kD
WebPhospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually … Webphospho-ERK (Thr202/Tyr204) Cellular Kit [CAT#: NRP-0322-P2169] This kit is designed for robust quantification of ERK modulation, phosphorylated at Thr202/Tyr204. ... The Total …
Webwhen phosphorylated at Thr202 and Tyr204. Upon activation, ERK 1/2 is phosphorylated and after lysis of the cell membrane, phospho-ERK1/2 (Thr202/Tyr 204) can be detected using the kit reagents. FRET signal. phospho-ERK1/2 phospho-ERK1/2. Phospho-ERK1/2 . Eu Cryptate antibody. Phospho-ERK1/2 d2 antibody. Figure 1: Principle of HTRF sandwich …
WebWestern blot analysis of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from EGF-treated A549 cells and PDGF-treated NIH/3T3 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Mouse mAb (1:500 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb (1:500 diluted,lower). Storage/Stability 2-8°C (short-term); -20°C (long-term) Expiration: hunnewell\u0027s cottages avon park flWebDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. Subcellular Location: Cytoplasm. Nucleus. Membrane>Caveola. Note: Autophosphorylation at Thr-207 promotes nuclear localization. marty kearyWebERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. hunn funeral home checotah okWebDetect and quantitate phospho-ERK in cell lysates using simple LANCE Ultra TR-FRET assays. ... High-Throughput SARS-COV-2 Testing Robotic Arms Scheduling & Control … marty kingston monctonWebIP result of anti-Phospho-ERK1/2 (Thr202/Tyr204) (IP:28733-1-AP, 2ug; Detection:28733-1-AP 1:1000) with Calyculin A treated PC-3 cells lysate 1552 ug. Save time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits $299 / 100 μL Cat No. 28733-1-AP In stock. Order within 29 hours and 16 mins to receive by Tuesday, April 4 marty kiar broward county floridaWebERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates. Interaction marty kiar broward countyWebERK1/2 signaling is activated in response to numerous extracellular stimuli including mitogens, growth factors, and cytokines. The primary activators of ERK1/2 are MEK1 and … marty king facebook