Hfq protein database
WebHfq is a bacterial RNA-binding protein that participates in post-transcriptional control of gene expression by facilitating the interactions between small non-coding RNAs and their target mRNAs. WebHfq comprises between 70 and 110 amino acids and forms homohexamers. It is an abundant protein, which is present in up to 60 000 monomers/ E. coli cell in the stationary phase. The major fraction is associated with the ribosomes, whereas a minor Hfq fraction appears to be associated with the nucleoid.
Hfq protein database
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WebThe Hfq protein was discovered in Escherichia coli as a host factor for bacteriophage Qβ RNA replication. Subsequent studies indicated that Hfq is a pleiotropic regulator of … Web29 set 2010 · The Hfq protein is reported to be an RNA chaperone, which is involved in the stress response and the virulence of several pathogens. In E. coli, Hfq can mediate the interaction between some sRNAs and their target mRNAs. But it is controversial whether Hfq plays an important role in S. aureus. In this study, we found that the deletion of hfq …
Web14 ott 2014 · The crystal structure of RydC in complex with E. coli Hfq at a 3.48 Å resolution illuminates how the protein interacts with and presents the sRNA for target recognition. Consolidating the protein-RNA complex is a host of distributed interactions mediated by the natively unstructured termini of Hfq. Based on the structure and other data, we ... Web1 ott 2024 · Hfq is a RNA-binding protein that plays a pivotal role in the control of gene expression in bacteria by stabilizing sRNAs and facilitating their pairing with multiple …
Web18 feb 2024 · Role of Hfq protein in cell growth. There have been reports that the host factor for phage Q β (Hfq) is closely related to cell growth [].For example, Hfq protein is … WebThe Hfq protein was discovered in Escherichia coli as a host factor for bacteriophage Qβ RNA replication. Subsequent studies indicated that Hfq is a pleiotropic regulator of bacterial gene expression. The regulatory role of Hfq is ascribed mainly to its function as an RNA-chaperone, facilitating interactions between bacterial non-coding RNA and its mRNA target.
WebHfq is a bacterial master regulator which promotes the pairing of nucleic acids. Due to the high molecular weight of the complexes formed between nucleic acids and the amyloid form of the protein, it is difficult to analyze solely by a gel shift assay the complexes formed, as they all migrate at the same position in the gel. In addition, precise kinetics …
WebRNA-binding protein Hfq. Gene. hfq. Status. UniProtKB reviewed (Swiss-Prot) Organism. Bacillus subtilis (strain 168) Amino acids. 73. Protein existence. Evidence at protein level. Annotation score. 2/5. ... Enzyme and pathway databases. BioCyc. BSUB:BSU17340-MON; Names & Taxonomy. Protein names. Recommended name. RNA-binding protein Hfq … clerk of courts marathon floridaWeb1 mag 2024 · The reads which mapped to rRNA database were removed by the short sequence alignment tool Bowtie2 ... Meanwhile, 11 GO categories significantly enriched in BP were related to biosynthesis and metabolism, protein modification, amino acid metabolic process ... including luxS, rpoN, hfq, cysE, and tarA, were down‐regulated ... clerk of courts maricopaWeb5 mar 2024 · Protein expression, purification and site-directed mutagenesis. Escherichia coliHfq was purified using the IMPACT-CN system as described previously . Briefly, E. coli Hfq (residues 2–69) was overexpressed in E. coli … bluish gray hex codeWeb15 mar 2024 · Introduction of an hfq-gfp allele at the native chromosomal locus restored motility to levels more comparable to that of the wild-type strain (SI Appendix, Fig. S1A), and immunoblot analysis detected a protein of the predicted size for Hfq-GFP (SI Appendix, Fig. S1B), indicating that a full-length, functional Hfq-GFP protein is produced in this strain. bluish gray floor tileWeb8 nov 2024 · AU-rich elements in 5’ untranslated region (UTR) are known to increase translation efficiency by recruiting S1 protein that facilitates the assembly of ribosomes. However, AU-rich elements also serve a binding site for Hfq protein, RNase E, etc. To investigate their roles in translation, mRNAs containing either an AU-rich element, … clerk of courts maricopa azWebHfq is a bacterial RNA-binding protein that participates in post-transcriptional control of gene expression by facilitating the interactions between small non-coding RNAs and their … bluish gray marker robloxWeb15 giu 2024 · The browser enables search queries and retrieval of primary sequences for any annotated feature in the database to serve as a resource for the C. difficile community. ... Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile. J. Bacteriol. 196, 3234–3248 (2014). Crossref. PubMed. clerk of courts marathon wi