WebThe Janelia Fluor® HaloTag® Ligands enable characterization of HaloTag® fusions in endogenous cellular settings. The Janelia Fluor® dyes 525, 585 and 635 are from the same family as Janelia Fluor® 549 and … WebFor Halo domain labeling, fluorescent Halo ligands (JF549 and JF646) were mixed in protein samples at ratio of 8:1 and reacted at room temperature for 30 min followed by incubation at 4 °C overnight. The dCas9-Halo with an aldehyde tag was fluorescently labeled with Cy5 hydrazide (GE Healthcare) as described (13).
Quantification of very low-abundant proteins in bacteria …
WebJF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor® products are … WebAug 31, 2015 · dCas9 Fluorescent Labeling. For Halo domain labeling, fluorescent Halo ligands (JF549 and JF646) were mixed in protein samples at ratio of 8:1 and reacted at room temperature for 30 min followed by incubation at 4 °C overnight. The dCas9-Halo with an aldehyde tag was fluorescently labeled with Cy5 hydrazide (GE Healthcare) as … northern ford cut bank
Choosing the right label for single-molecule tracking in live …
WebJun 21, 2024 · We stained the EWS/FLI1-Halo knock-in A673 cells with two fluorescent ligands (40, 41): ... Snapshots of an SPT movie imaging endogenous EWS/FLI1-Halo labeled with two Halo ligands, JF549 (200 nM) and PA-JF646 (20 nM). We imaged the EWS/FLI1-Halo hubs in the JF549 channel (green) and tracked individual EWS/FLI1 … WebIntroducing PrentaLux. Performance-based products and hospitality minded. Mixing these elegant shapes, sizes, colors, and textures creates the best combination of aesthetics … WebApr 18, 2024 · For JF549/JF646 comparisons samples were stained with either Halo-JF646 and SNAP-JF549, or Halo-JF549 and SNAP-JF646, diluted to 0.5 μM in PBS (Grimm et al., 2015). Samples were stained at 37°C with shaking for 30 min. Samples were then washed 6x 10 min in PBT (0.1% Triton X-100) and mounted in Vectashield with DAPI. northern foreign auto parts